| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 143 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-200 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-SMC1 (Ser957) Rabbit Monoclonal Antibody detects endogenous levels of SMC1 protein only when phosphorylated at Ser957. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
SMC1 also known as SMC1α, SMC1A is a crucial 1,233 amino acid nuclear protein that plays a vital role in maintaining chromosome cohesion during the cell cycle. SMC1 is a key component of the cohesion complex, which is essential for the proper alignment and separation of sister chromatids following DNA replication. SMC1′s importance extends beyond its structural role; SMC1 interacts with BRCA1 and is phosphorylated by ATM, highlighting SMC1′s involvement in DNA repair mechanisms. Mutations in the gene encoding SMC1 have been linked to Cornelia de Lange syndrome (CdLS), a developmental disorder characterized by facial dysmorphia, upper limb malformations, and cognitive impairments. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells, using Phospho-SMC1 (Ser957) Rabbit Monoclonal Antibody (EAB21631) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 143 kDa; Observed band size: 160 kDa |
