| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 21 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IHC-P=1:50-200 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | RKIP Rabbit Monoclonal Antibody detects endogenous levels of RKIP protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
RKIP also known as PEBP1 is a member of the phosphatidylethanolamine-binding family of proteins and has been shown to modulate multiple signaling pathways, including the MAP kinase (MAPK), NF-kappa B, and glycogen synthase kinase-3 (GSK-3) signaling pathways. RKIP plays a crucial role in regulating the MAP kinase signaling pathway, which is essential for various cellular processes such as proliferation, differentiation, and apoptosis. RKIP is primarily located in the cytosol, where RKIP interacts with key signaling molecules, including the serine/threonine kinase Raf-1. By binding to both active and inactive forms of Raf-1, RKIP modulates the activity of downstream effectors like MEK and ERK, thereby influencing cellular responses to growth factors and stress signals. RKIP′s ability to inhibit MEK activation by competing with its substrate is particularly important, as this mechanism helps maintain the balance of signaling pathways that can lead to oncogenic transformation when dysregulated. |
| Product Image Gallery | |
 Western blot analysis of extracts on Siha, Mouse brain cells, using RKIP Rabbit Monoclonal Antibody (EAB21590) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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