| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC, FC | 113 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-200 IF/ICC=1:50-200 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Sodium Potassium ATPase Rabbit Monoclonal Antibody detects endogenous levels of Sodium Potassium ATPase protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Na+/K+-ATPase also known as the sodium-potassium pump is a crucial plasma membrane protein responsible for maintaining cellular homeostasis by actively transporting three sodium ions out of the cell and two potassium ions into the cell against their electrochemical gradients. This activity is essential for regulating cellular volume, maintaining the resting membrane potential, and facilitating secondary active transport of numerous solutes. Located in the plasma membrane, Na+/K+-ATPase plays a vital role in key physiological processes including nerve impulse transmission, muscle contraction, and kidney function. The protein complex comprises multiple isoforms of α, β, and γ subunits, where α subunit contains binding sites for ATP and transported ions, β subunit ensures proper folding and membrane localization, and γ subunit modulates enzyme′s ATP affinity in specific tissues. |
| Product Image Gallery | |
 Western blot analysis of extracts on A549 cells, using Sodium Potassium ATPase Rabbit Monoclonal Antibody (EAB21579) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 113 kDa; Observed band size: 100 kDa |
