| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IF/ICC | 54 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Chk1 Rabbit Monoclonal Antibody detects endogenous levels of Chk1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Chk1 also known as CHEK1 plays a critical role in the cellular response to DNA damage, functioning as a key regulator in the G2 DNA damage checkpoint. By phosphorylating Cdc25C, Chk1 inhibits its activity, which in turn prevents the activation of cyclin-dependent kinases and blocks the progression of mitosis. This regulatory mechanism is vital for maintaining genomic stability, as cells can repair DNA damage before entering mitosis, thereby preventing the propagation of damaged DNA to daughter cells. Chk1 has been shown to interact with other proteins involved in cell cycle regulation, including Wee1, further underscoring Chk1′s importance in the intricate network of cell cycle control. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat cells, using CHK1 Rabbit Monoclonal Antibody (EAB21574) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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