| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 102 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Dnmt3a Rabbit Monoclonal Antibody detects endogenous levels of Dnmt3a protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Dnmt3a plays a crucial role in the regulation of gene expression by catalyzing the addition of methyl groups to cytosines in the CpG dinucleotide context, which is essential for establishing and maintaining epigenetic marks during development and cellular differentiation. Dnmt3a is predominantly localized in the nucleus, where anti-Dnmt3a antibody (64B814) can detect interactions with DNA and other regulatory proteins that influence chromatin structure and gene accessibility. The proper functioning of Dnmt3a is vital for normal embryonic development, and dysregulation of its activity has been implicated in various diseases, including cancer. Dnmt3a works in concert with other DNA methyltransferases, such as Dnmt1 and Dnmt3b, to ensure the fidelity of DNA methylation patterns, which are critical for cellular identity and function. |
| Product Image Gallery | |
 Western blot analysis of extracts on HEK293, Mouse brain, Rat brain cells, using Dnmt3a Rabbit Monoclonal Antibody (EAB22580) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 102 kDa; Observed band size: 130 kDa |
