| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IF/ICC, FC, ELISA | 46 | Human, Mouse, Rat, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Aurora A Mouse Monoclonal Antibody detects endogenous levels of Aurora A protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Aurora A also known as ARK-1, AURKA is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. Aurora A plays a crucial role in cell division by regulating centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis. Proper Aurora A function is vital for maintaining genomic stability, as errors in these processes can lead to aneuploidy and contribute to tumorigenesis. Aurora A is primarily localized in the nucleus and associates with key components of the mitotic machinery, such as the centrosome and spindle poles, highlighting Aurora A′s importance in the orchestration of mitotic events. Notably, Aurora A expression is significantly elevated in various colon carcinoma cell lines, indicating Aurora A′s potential role as a biomarker for cancer progression. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7, HEK293 cells, using Aurora A Mouse Monoclonal Antibody (EAB21529) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunofluorescence analysis of Hela cell, using Aurora A Mouse Monoclonal Antibody (EAB21529)(green) at 1:100 dilution.
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