| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC, FC | 36 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IHC-P=1:50-200 IF/ICC=1:100-500 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | YB1 Rabbit Monoclonal Antibody detects endogenous levels of YB1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
YB1 also known as DNA-binding protein B, YBX1 belongs to a multifunctional protein family that plays a crucial role in regulating transcription and translation. YB1 can interact with diverse nucleic acid structures, functioning as both a transcription factor and an mRNA masking protein. A modular structure featuring a highly conserved N-terminal cold-shock domain and four basic C-terminal domains rich in arginine clusters and aromatic residues enables YB1′s binding capabilities. YB1 drives cell proliferation by activating growth-associated gene expression while repressing cell death-associated FAS gene expression, demonstrating a vital role in maintaining cellular homeostasis through regulation of cell survival and apoptosis pathways. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat cells, using YB1 Rabbit Monoclonal Antibody (EAB21523) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:10000 dilution.
Predicted band size: 36 kDa; Observed band size: 50 kDa |
