| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC, FC | 211 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MLYK Rabbit Monoclonal Antibody detects endogenous levels of MLYK protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MYLK also known as MLCK is a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. MYLK protein plays a crucial role in muscle contraction by phosphorylating myosin regulatory light chains, which facilitates interaction between myosin and actin filaments, leading to contractile activity. This function is particularly important in smooth muscle tissues, where MYLK isoforms contribute to regulation of vascular tone and gastrointestinal motility. MYLK gene encodes several isoforms, including those specific to smooth muscle and non-muscle tissues, as well as telokin, a small isoform that stabilizes unphosphorylated myosin filaments. Multiple transcript variants for MYLK gene highlight diverse regulatory roles across different tissues. |
| Product Image Gallery | |
 Western blot analysis of extracts on Human lung, Rat lung cells, using MYLK Rabbit Monoclonal Antibody (EAB21520) at 1:3000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 211 kDa; Observed band size: 110 kDa |
