| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC, FC | 58, 61, 62 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IHC-P=1:50-200 IF/ICC=1:50-200 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-PAK1/2/3 (Ser144/141/139) Rabbit Monoclonal Antibody detects endogenous levels of PAK1/PAK2/PAK3 only when phosphorylated at Ser144/Ser141/Ser139. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
The alpha, beta, and gamma PAK isoforms, which are serine/threonine kinases, play a crucial role in cellular signaling pathways by interacting with the small GTPases Rac1 and Cdc42. These interactions are vital for regulating cytoskeletal dynamics and cell motility, processes that are essential for various physiological functions, including cell migration, proliferation, and differentiation. Notably, γPAK has eight autophosphorylation sites, with phosphorylation at Ser 141 and Thr 402 being particularly important for activation. Upon activation, γPAK can dissociate from Rac1 and Cdc42 complex, allowing phosphorylation of downstream substrates such as Mek kinase, which is a key player in the JNK signaling pathway. This intricate regulation of signaling pathways underscores the importance of PAK proteins in maintaining cellular homeostasis and responding to external stimuli. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, NIH/3T3 cells, using Phospho-PAK1/2/3 (Ser144/141/139) Rabbit Monoclonal Antibody (EAB21515) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 58,61,62 kDa; Observed band size: 65 kDa |
