| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC, FC | 37 | Human, Mouse, Rat, Zebrafish |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Lactate Dehydrogenase/LDH Rabbit Monoclonal Antibody detects endogenous levels of Lactate Dehydrogenase/LDH protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | P00338, P07195, P07864 |
| Entrez Gene ID | 3939, 3945, 3948 |
| Product Description | |
Lactate Dehydrogenase/LDH plays a crucial role in cellular metabolism by catalyzing the conversion of L-lactate and NAD to pyruvate and NADH, marking the final step of anaerobic glycolysis. This enzymatic activity is vital for energy production, especially in tissues that rely heavily on anaerobic metabolism, such as muscle during intense exercise. Each LDH isoform exhibits distinct tissue distribution, with LDH-A predominantly found in muscle, LDH-B in the heart, and LDH-C restricted to the testis and sperm, making these isoforms important markers for various physiological and pathological conditions, including germ cell tumors. The genes encoding human LDH-A and LDH-C are located on chromosome 11, while the LDH-B gene is found on chromosome 12, and deficiencies in the LDH-A gene have been associated with exertional myoglobinuria, highlighting the clinical significance of LDH in metabolic disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7, A549 cells, using Lactate Dehydrogenase/LDH Rabbit Monoclonal Antibody (EAB21514) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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