| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP | 46 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | TPBG/5T4 Rabbit Monoclonal Antibody detects endogenous levels of TPBG/5T4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
TPBG(Trophoblast glycoprotein) also known as 5T4 is a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. TPBG expression has been linked to cytoskeletal remodeling and signaling programs that influence epithelial–mesenchymal transition–like behaviors, including modulation of Wnt/β-catenin–associated processes and cell migration pathways. In human tissues and disease models, altered TPBG levels are frequently used as a marker of invasive cellular phenotypes, supporting investigations into mechanisms underlying tumor progression and metastasis-associated biology. Its membrane localization and association with migratory phenotypes make TPBG a useful node for studying cell–cell interactions and microenvironmental responses. |
| Product Image Gallery | |
 Western blot analysis of extracts on SKBR3, MCF7 cells, using TPBG/5T4 Rabbit Monoclonal Antibody (EAB21502) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 46 kDa; Observed band size: 72-80 kDa |
