| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC | 84 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | B-Raf Mouse Monoclonal Antibody detects endogenous levels of B-Raf protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
B-Raf also known as Raf-B, BRAF plays a crucial role in regulating cell growth and differentiation by acting as a key component in the mitogen-activated protein kinase (MAPK) signaling pathway. This pathway is vital for transmitting signals from cell surface receptors to the nucleus, ultimately influencing cellular responses to growth factors and stress. Raf-B is predominantly located in the cytoplasm, where Raf-B interacts with other signaling molecules, including ERK/MAP kinases and ribosomal S6 kinase, to propagate growth signals. Notably, Raf-B expression is highly restricted, with the highest levels found in the cerebrum and testis, indicating specialized functions in these tissues. Raf-B regulation is critical, as unregulated Raf-B has been associated with oncogenic transformation, making Raf-B a significant target for cancer research and therapeutic interventions. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HepG2, NIH/3T3 cells, using B-Raf Mouse Monoclonal Antibody (EAB21501) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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