| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC, FC | 23 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 IHC-P=1:50-200 IF/ICC=1:50-200 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | DARPP-32 Rabbit Monoclonal Antibody detects endogenous levels of DARPP-32 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
DARPP-32 also known as PPP1R1B is a bifunctional signal transduction molecule. DARPP-32 plays a crucial role in dopaminergic signaling pathways, which are vital for numerous brain functions, including motor control, reward processing, and cognitive functions. Located predominantly in neurons that receive dopaminergic input, DARPP-32 acts as a key integrator of dopamine signaling, where phosphorylation state is critical for modulating the activity of protein phosphatase 1, a major enzyme involved in synaptic plasticity and memory formation. Phosphorylation of DARPP-32 on threonine is stimulated by dopamine binding to D1-like receptors, while D2-like receptor activation leads to dephosphorylation, highlighting the intricate balance of signaling pathways that govern neuronal function. |
| Product Image Gallery | |
 Western blot analysis of extracts on Mouse brain, Rat brain cells, using DARPP-32 Rabbit Monoclonal Antibody (EAB21493) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 23 kDa; Observed band size: 32 kDa |
