| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 532 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Plectin Rabbit Monoclonal Antibody detects endogenous levels of Plectin protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Plectin also known as PLEC is a crucial cytoskeletal protein that plays a significant role in maintaining cellular integrity by crosslinking intermediate filaments to microtubules and microfilaments, thereby stabilizing the cytoplasm. Plectin′s ability to anchor intermediate filaments to both plasma and nuclear membranes is vital for cellular architecture and function. Plectin interacts with key proteins such as Lamin B and vimentin, with these interactions being modulated by various protein kinases. For instance, phosphorylation by protein kinase A or protein kinase C reduces binding affinity to Lamin B, while phosphorylation by protein kinase A enhances interaction with vimentin. Plectin serves as a substrate for p34cdc2 kinase, highlighting involvement in cell cycle regulation. |
| Product Image Gallery | |
 Western blot analysis of extracts on A431, U2OS, Rat brain cells, using Plectin Rabbit Monoclonal Antibody (EAB22354) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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