| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 65 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Paxillin Rabbit Monoclonal Antibody detects endogenous levels of Paxillin protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Paxillin also known as PXN is a focal adhesion phosphoprotein localized to the cytoskeleton. Paxillin plays a pivotal role in cell adhesion, migration, and signal transduction pathways. Paxillin′s interaction with focal adhesion kinase (FAK) is particularly important, as this interaction is essential for regulating tyrosine phosphorylation in response to cell adhesion. Phosphorylation of paxillin is a key event in signaling pathways activated by various growth factors and oncogenes, which can lead to significant changes in cell behavior, including migration and proliferation. Dynamic regulation of paxillin through tyrosine phosphorylation is vital for reorganizing the actin cytoskeleton, which is critical for maintaining cell shape and motility. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Raw264.7 cells, using Paxillin Rabbit Monoclonal Antibody (EAB21455) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
