| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 26 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CD63 Mouse Monoclonal Antibody detects endogenous levels of CD63 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CD63 also known as LAMP3, TSPAN30 is a member of the tetraspanin family of proteins characterized by four transmembrane domains, which are essential for the formation of multi-protein complexes involved in cell signaling and adhesion. The structural integrity provided by these transmembrane regions is crucial for CD63′s role in organizing membrane microdomains and interacting with specific integrins, thereby influencing cellular processes such as migration, proliferation, and immune responses. Predominantly located within lysosomal membranes, CD63 translocates to the plasma membrane upon cellular activation, such as during platelet activation, where CD63 plays a key role in phagocytosis and the fusion of lysosomes with phagosomes. This dynamic localization is vital for maintaining cellular homeostasis and effective immune function. |
| Product Image Gallery | |
 Western blot analysis of extracts on A375 cells, using CD63 Mouse Monoclonal Antibody (EAB21453) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 26 kDa; Observed band size:30-65 kDa |
