| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 351 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-200 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ATM Rabbit Monoclonal Antibody detects endogenous levels of ATM protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ATM is a crucial member of the phosphatidylinositol 3-kinase-related kinase (PIKK) family, distinguished by its complex structure that includes multiple HEAT repeats and a central kinase domain. This intricate architecture is essential for ATM′s role in orchestrating the cellular response to DNA double-strand breaks, facilitating DNA repair, cell cycle control, and apoptosis to maintain genomic stability. Understanding the structure of ATM is vital as ATM′s ability to interact with various substrates and regulatory proteins modulates critical signaling pathways involved in cellular homeostasis and stress responses. Mutations in the ATM gene are responsible for the autosomal recessive disorder ataxia telangiectasia, which is characterized by neurodegeneration, immune deficiencies, and an increased predisposition to cancer, highlighting ATM′s significance in normal cellular function and disease. |
| Product Image Gallery | |
 Western blot analysis of extracts on CRC, Hela cells, using ATM Rabbit Monoclonal Antibody (EAB21430) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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