| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, FC | 114 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:10-100 FC=1:10-100 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | RNF20 Rabbit Monoclonal Antibody detects endogenous levels of RNF20 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
RNF20 (ring finger protein 20) also known as BRE1, BRE1A, hBRE1 is a 975 amino acid nuclear protein that belongs to the BRE1 family. As a component of the RNF20/40 complex, RNF20 functions as an E3 ubiquitin-protein ligase that regulates the monoubiquitination and subsequent degradation of select residues on target proteins, such asf Histone H2B. RNF20 is required for transcriptional activation of Hox genes and is most likely recruited by p53 to the MDM2 promoter, thereby acting as a transcriptional co-activator. RNF20 contains one zinc finger domain and exists as a homodimer |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7, Jurkat cells, using RNF20 Rabbit Monoclonal Antibody (EAB21408) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
