| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 21 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PUMA Rabbit Monoclonal Antibody detects endogenous levels of PUMA protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PUMA also known as JFY1, BBC3 is a member of the BCL-2 family of proteins. PUMA plays a critical role in regulating apoptosis, particularly in response to cellular stressors such as γ-radiation and glucocorticoids. PUMA is a mitochondrial pro-apoptotic Bcl-2 homology domain (BH3)-only protein that initiates apoptosis through a Bax- and mitochondria-dependent pathway, making PUMA essential for maintaining cellular homeostasis and preventing tumorigenesis. PUMA gene encodes several splice variants, including PUMAα, β, γ, and δ, with PUMAα and PUMAβ containing the crucial BH3 domain that facilitates interaction with anti-apoptotic proteins like Bcl-2 and Bcl-xL. This interaction is vital for the pro-apoptotic function of PUMA, as PUMA helps transmit death signals to the mitochondria, thereby promoting cell death in lymphoid cells. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, THP-1, C6 cells, using PUMA Rabbit Monoclonal Antibody (EAB21374) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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