| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 81 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:20-200 IHC-P=1:50-200 IF/ICC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | VCAM1 Rabbit Monoclonal Antibody detects endogenous levels of VCAM1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
VCAM1,also known as CD106 is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. VCAM1 consists of seven extracellular immunoglobulin-like domains, a single transmembrane segment, and a short cytoplasmic tail; this structure is crucial as the immunoglobulin-like domains facilitate binding to integrins on leukocytes, particularly VLA-4 (α4β1 integrin). VCAM1 binding mediates the adhesion of leukocytes to the vascular endothelium, a key step in immune surveillance and inflammatory response. VCAM1 expression on endothelial cells is upregulated by inflammatory cytokines such as interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-α), and lipopolysaccharide (LPS). Understanding VCAM1 structural features is important because alterations can affect integrin interaction, impacting leukocyte adhesion and migration, which are critical in conditions like atherosclerosis and autoimmune diseases. |
| Product Image Gallery | |
 Western blot analysis of extracts on NIH/3T3, Rat spleen cells, using VCAM1 Rabbit Monoclonal Antibody (EAB21319) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Lane1: NIN/3T3 Lane2: Human spleen Predicted band size: 81 kDa; Observed band size: 100 kDa |
