| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IF/ICC, ELISA | 42 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Erk2 Rabbit Polyclonal Antibody detects endogenous levels of Erk2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ERK2 also known as P42, MAPK1 is a member of the MAP kinase family. ERK2 is a pivotal component of the MAPK/ERK signaling pathway, which transmits extracellular signals from growth factors, hormones, and neurotransmitters to elicit cellular responses. Activation of ERK2 occurs via phosphorylation by upstream kinases like MEK1/2, leading to dimerization and translocation into the nucleus. Once in the nucleus, ERK2 phosphorylates various transcription factors and regulatory proteins, influencing gene expression related to cell proliferation, differentiation, survival, and development. Dysregulation of ERK2 activity has been implicated in numerous pathological conditions, including cancer and neurodegenerative diseases, making ERK2 a significant focus in biomedical research. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, MCF7, A431, NIH/3T3 cells, using Erk2 Mouse Monoclonal Antibody (EAB14779) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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