| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 37 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Bmi1 Mouse Monoclonal Antibody detects endogenous levels of Bmi1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Bmi1 is a ring finger protein that is major component of the polycomb group complex 1 (PRC1). Bmi1 protein plays a crucial role in cellular processes, particularly in gene expression regulation and stem cell properties maintenance. Bmi1 is primarily located in the nucleus, where Bmi1 functions as a transcriptional repressor, interacting with chromatin and influencing expression of genes involved in cell cycle regulation and development. Nuclear localization allows Bmi1 to modulate transcription of target genes, including those associated with oncogenesis and stem cell maintenance. Bmi1 has been implicated in repression of the Ink4a/Arf locus, which is essential for controlling cellular senescence and tumor suppression. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, 293T, Jurkat, PC12, Mouse kidney cells, using Bmi1 Mouse Monoclonal Antibody (EAB14765) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 37 kDa; Observed band size: 40 kDa |
