| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 74 | Human, Mouse, Rat, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MMP-2 Rabbit Polyclonal Antibody detects endogenous levels of MMP-2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MMP-2 is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. MMP-2 plays a crucial role in degrading ECM components including collagen types IV, V, VII, and X, as well as gelatin type I. MMP-2′s extracellular localization enables tissue remodeling, angiogenesis, and wound healing by facilitating ECM barrier breakdown and cellular migration. This localization becomes critical in cancer metastasis, where MMP-2-mediated ECM degradation enables cancer cell invasion and spread. MMP-2 also modulates bioactive ECM molecules, influencing cell signaling pathways governing proliferation, differentiation, and apoptosis. Ras signaling pathway regulates MMP-2 activation and secretion, which require calcium and zinc ions for catalytic activity. |
| Product Image Gallery | |
 Western blot analysis of extracts on HCT116, SW480, PC3 cells, using MMP-2 Rabbit Polyclonal Antibody (EAB10163) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human lung tissue, using MMP-2 Rabbit Polyclonal Antibody (EAB10163) at 1:200 dilution(4° overnight).
 Immunofluorescence analysis of Hela cell, using MMP-2 Rabbit Polyclonal Antibody (EAB10163)(green) at 1:200 dilution(4° overnight).
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