| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 68 | Human, Mouse, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-SF1 (Ser82) Rabbit Polyclonal Antibody detects endogenous levels of SF1 only when phosphorylated at Ser82. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
SF1(Splicing factor 1) also known as ZFP162, ZFM1 is a nuclear pre-mRNA splicing factor. SF1 plays a crucial role in the splicing process by specifically recognizing the seven-nucleotide branch point sequence located at 3′ splice sites, which is essential for the assembly of early spliceosomal complexes. This function is vital for accurate processing of pre-mRNA, influencing gene expression and cellular function. SF1 acts as a transcriptional repressor, potentially regulating proliferation and expression of pro-inflammatory gene products in smooth muscle cells. Expression of SF1 can be downregulated by cytokines, which may contribute to pathogenesis of hyperproliferative inflammatory diseases. SF1 includes a nuclear transport domain, a metal-binding motif, and regions rich in glutamine and proline, which are important for functional interactions. |
| Product Image Gallery | |
 Western blot analysis of extracts on HuvEc cells treated with Anisomycin, using Phospho-SF1 (Ser82) Rabbit Polyclonal Antibody (EAB14743) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
 Immunohistochemistry analysis of paraffin-embedded Human brain tissue, using Phospho-SF1 (Ser82) Rabbit Polyclonal Antibody (EAB14743) at 1:100 dilution.
 Immunofluorescence analysis of Hela cell, using Phospho-SF1 (Ser82) Rabbit Polyclonal Antibody (EAB14743) at 1:100 dilution.
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