| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 131 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-HDAC6 (Ser22) Rabbit Polyclonal Antibody detects endogenous levels of HDAC6 protein only when phosphorylated at Ser22. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HDAC6(Histone deacetylase 6) is a member of the histone deacetylase family. HDAC6 plays a crucial role in regulating cellular processes by deacetylating non-histone proteins, which influences various signaling pathways and cellular functions. HDAC6 recognizes a protein involved in cytoskeleton regulation and essential for proper microtubule functioning, which are vital for cell shape, motility, and intracellular transport. HDAC6′s ability to interact with and deacetylate tubulin highlights its importance in maintaining cellular architecture and function. Dysregulation of HDAC6 has been implicated in several diseases, including cancer and neurodegenerative disorders, making HDAC6 a significant target for therapeutic intervention. |
| Product Image Gallery | |
 Western blot analysis of extracts on NIH/3T3 cells treated with Anisomycin, using Phospho-HDAC6 (Ser22) Rabbit Polyclonal Antibody (EAB14301) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
 Immunohistochemistry analysis of paraffin-embedded Human testis tissue, using Phospho-HDAC6 (Ser22) Rabbit Polyclonal Antibody (EAB14301) at 1:200 dilution.
 Immunofluorescence analysis of HepG2 cell, using Phospho-HDAC6 (Ser22) Rabbit Polyclonal Antibody (EAB14301) at 1:200 dilution.
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