| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 122 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | HDAC5 Rabbit Polyclonal Antibody detects endogenous levels of HDAC5 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HDAC5(Histone deacetylase 5) is a member of the histone deacetylase family. HDAC5 is predominantly located in the nucleus but can shuttle between the nucleus and cytoplasm in response to cellular signals, a process regulated by phosphorylation and interaction with 14-3-3 proteins. This subcellular localization is crucial because, within the nucleus, HDAC5 deacetylates histone proteins, leading to a condensed chromatin structure and transcriptional repression of specific genes. Dynamic movement between cellular compartments allows HDAC5 to modulate gene expression, impacting processes like muscle differentiation, neuronal function, and stress responses. Dysregulation of HDAC5 localization or function has been implicated in various diseases, including cancer and cardiac hypertrophy, highlighting HDAC5′s importance as a therapeutic target. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562, NIH/3T3 cells, using HDAC5 Rabbit Polyclonal Antibody (EAB14584) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry of paraffin-embedded Human tonsil tissue, using HDAC5 Rabbit Polyclonal Antibody (EAB14584) at 1:100 dilution.
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