| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IF/ICC, ELISA | 48 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-GATA-3 (Ser308) Rabbit Polyclonal Antibody detects endogenous levels of GATA-3 protein only when phosphorylated at Ser308. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
GATA-3 is a protein which belongs to the GATA family of transcription factors. GATA-3 plays a crucial role in the development and function of T lymphocytes, acting as a transcription factor that regulates the expression of genes essential for T cell differentiation and function. GATA-3 expression is particularly important in the immune response, as GATA-3 is involved in the development of Th2 cells, which are critical for orchestrating responses to extracellular pathogens and in allergic reactions. GATA-3 is predominantly located in the nucleus of T cells, where GATA-3 binds to specific DNA sequences to modulate gene expression. Nuclear localization is vital for function, as direct influence on the transcription of target genes drives T cell development and function. GATA-3 regulation of these processes underscores significance in both normal immune function and in the pathogenesis of various diseases, including allergies and autoimmune disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on Mosue spleen cells, using Phospho-GATA-3 (Ser308) Rabbit Polyclonal Antibody (EAB14593) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunofluorescence analysis of extracts on HuvEc cells, using Phospho-GATA-3 (Ser308) Rabbit Polyclonal Antibody (EAB14593) at 1:100 dilution.
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