| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IF/ICC, ELISA | 79 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-b-Myb (Ser577) Rabbit Polyclonal Antibody detects endogenous levels of b-Myb only when phosphorylated at Ser577. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
b-Myb also known as MYBL2 is a member of the MYB family of transcription factor genes, is a nuclear protein involved in cell cycle progression. b-Myb plays a crucial role as a transcription factor, particularly in the regulation of genes involved in cell cycle progression and differentiation. b-Myb expression remains low or undetectable in quiescent cells but significantly increases during the G1/S-phase transition following mitogenic stimulation. This regulation allows b-Myb to facilitate the transition from a resting state to active cell division, thereby influencing cellular proliferation. Studies have indicated that b-Myb can rescue cells from p53-induced G1 arrest mediated by p21, highlighting B-Myb′s importance in overcoming cell cycle checkpoints that would otherwise prevent proliferation in response to stress signals. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562 cells, using Phospho-b-Myb (Ser577) Rabbit Polyclonal Antibody (EAB14691) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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