| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 55 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Fumarase/FH Mouse Monoclonal Antibody detects endogenous levels of Fumarase/FH protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
FH(Fumarate hydratase) also known as Fumarate is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. FH plays a significant role in the Krebs cycle by catalyzing the reversible hydration of fumaric acid to produce l-malic acid. This enzymatic activity is vital for cellular respiration and energy production, highlighting the importance of FH in metabolic processes. Notably, germline mutations in the FH gene are linked to a predisposition for renal defects, including multiple cutaneous and uterine leiomyoma syndrome, as well as an increased risk of renal and smooth muscle tumors, although they do not correlate with prostate cancer. Additionally, FH deficiency has been associated with neurological complications, such as seizures, stemming from prenatal brain dysgenesis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HepG2, 293T cells, using Fumarase/FH Mouse Monoclonal Antibody (EAB11971) at 1:3000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 55 kDa; Observed band size: 50 kDa |
