| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC | 70 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | AFP Mouse Monoclonal Antibody detects endogenous levels of AFP protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
AFP is a vital glycoprotein predominantly synthesized in the fetal liver, where it plays a crucial role in transporting essential ligands such as copper, nickel, and fatty acids within the cell and extracellular environment. In adult tissues, AFP is typically present only in trace amounts; however, its expression is significantly elevated in hepatocellular carcinoma and other liver-related cancers, making AFP a critical biomarker for early cancer detection and monitoring tumor progression. The cellular localization of AFP, primarily within the cytoplasm and secreted into the extracellular matrix, is important for its function in ligand transport and its involvement in cancer cell growth and metastasis. Additionally, the regulation of AFP expression is controlled by key transcription factors including HNF-1, which promotes AFP expression, and NF-1, which inhibits its promoter activity. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2 cells, using AFP Mouse Monoclonal Antibody (EAB14763) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human hepato caricnoma tissue, using AFP Mouse Monoclonal Antibody (EAB14763) at 1:500 dilution.
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