| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 26 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Galectin-3 Rabbit Polyclonal Antibody detects endogenous levels of Galectin-3 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Galectin-3 also known as LGALS3 is a member of the galectin family of soluble β-galactoside-binding lectins characterized by a single carbohydrate recognition domain (CRD) that is crucial for binding to galactose-containing glycoconjugates. Structurally, galectin-3 can exist in both monomeric and oligomeric forms, allowing galectin-3 to engage in diverse interactions that are essential for modulating cell-to-cell adhesion, cell-to-extracellular matrix (ECM) interactions, and various cellular signaling pathways. Post-translational modifications of galectin-3, such as phosphorylation and oxidation, play a vital role in regulating function, stability, and localization within the cell, thereby influencing processes like apoptosis, immune responses, and cancer progression. These modifications can alter galectin-3′s ability to interact with other proteins and carbohydrates, thereby affecting cellular homeostasis and disease states. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells, using Galectin-3 Rabbit Polyclonal Antibody (EAB14652) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
