| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 85 | Human, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MLH1 Mouse Monoclonal Antibody detects endogenous levels of MLH1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MLH1 (MutL homolog 1) plays a crucial role in the DNA mismatch repair (MMR) process, which is vital for maintaining genetic stability by correcting DNA replication errors. MLH1 helps identify deficiencies linked to various human cancers, particularly due to mutations or epigenetic silencing of the MLH1 gene. MLH1 is predominantly located in the nucleus, where MLH1 forms a complex with other MMR proteins to identify and repair mismatched DNA bases, thereby preventing mutations that could lead to tumorigenesis. MLH1 expression is notably observed in various tissues, including lymphocytes, heart, colon, breast, lung, spleen, testis, prostate, thyroid, and gall bladder, with MLH1 methylation being a common feature in several ovarian tumors. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, A549, MCF7 cells, using MLH1 Mouse Monoclonal Antibody (EAB21725) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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