| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 49 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-LKB1 (Ser428) Rabbit Polyclonal Antibody detects endogenous levels of LKB1 only when phosphorylated at Ser428. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
LKB1 also known as STK11 is a serine/threonine kinase that regulates cell polarity and energy metabolism and functions as a tumor suppressor. LKB1 plays a pivotal role in regulating cellular energy homeostasis, cell polarity, and metabolism by activating AMP-activated protein kinase (AMPK) and other related kinases. The structure of LKB1 includes a conserved kinase domain essential for enzymatic activity, as well as regulatory regions that facilitate interaction with other proteins and substrates. Post-translational modifications of LKB1, such as phosphorylation and ubiquitination, are critical for modulating activity, stability, and localization within the cell, thereby influencing ability to act as a tumor suppressor. Mutations and alterations in LKB1 are strongly associated with Peutz-Jeghers Syndrome (PJS), a hereditary disorder characterized by benign polyps in the gastrointestinal tract, pigmented spots on the lips, and an increased risk of various cancers. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells treated with PMA, using Phospho-LKB1 (Ser428) Rabbit Polyclonal Antibody (EAB14549) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 49 kDa; Observed band size: 65 kDa  Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue, using Phospho-LKB1 (Ser428) Rabbit Polyclonal Antibody (EAB14549) at 1:100 dilution.  Immunofluorescence analysis of NIH/3T3 cell, using Phospho-LKB1 (Ser428) Rabbit Polyclonal Antibody (EAB14549) at 1:100 dilution.
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