| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IF/ICC, ELISA | 32 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MARCKS Rabbit Polyclonal Antibody detects endogenous levels of MARCKS protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MARCKS also known as 80K or 80K-L is a substrate for protein kinase C. It is localized to the plasma membrane and is an actin filament crosslinking protein. plays a crucial role as a major substrate for protein kinase C, which is vital for numerous cellular processes, including cell signaling and cytoskeletal organization. The human MARCKS protein consists of 332 amino acids and is primarily located at the plasma membrane, where MARCKS can rapidly dissociate upon phosphorylation by different protein kinase C isoforms, such as PKC α and PKC ε, which are particularly active in NIH/3T3 fibroblasts. This dynamic regulation of MARCKS is essential for its function in cellular signaling pathways. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells, using MARCKS Rabbit Polyclonal Antibody (EAB14456) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
