| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 33 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | NPM Rabbit Polyclonal Antibody detects endogenous levels of NPM protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
NPM also known as NPM1, B23 is a multifunctional protein, plays a crucial role in ribosomal assembly and ribosomal RNA transport, essential for protein synthesis in cells. NPM′s nucleolar localization is particularly important for ribosome biogenesis, and disruption in this process can lead to impaired cellular function and contribute to diseases such as cancer. NPM undergoes significant post-translational modifications, including phosphorylation by Cdc2 kinase during mitosis, which regulates activity and protein interactions. NPM′s interaction with various proteins, including karyopherin α and karyopherin β, highlights a role in nuclear transport, making NPM vital for maintaining cellular homeostasis and function. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HT29, NIH/3T3 cells, using NPM Rabbit Polyclonal Antibody (EAB10981) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using NPM Rabbit Polyclonal Antibody (EAB10981) at 1:100 dilution.
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