| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 32 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-p57 (Thr310) Rabbit Polyclonal Antibody detects endogenous levels of p57 only when phosphorylated at Thr310. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
p57 also known as Kip2 is a member of the p21 Waf1/Cip1/p27 family of cyclin-dependent kinase inhibitors, playing a significant role in regulating cell cycle progression by inhibiting several G1 cyclin/Cdk complexes. This inhibition is vital for maintaining proper cell cycle control, particularly in response to cellular stress or DNA damage, as p57 prevents premature progression into the S phase, thereby allowing time for DNA repair mechanisms to function effectively. Notably, p57 is not regulated by p53, which distinguishes p57 from other family members like p21 Waf1/Cip1, and p57 overexpression can lead to a robust G1 arrest, highlighting p57′s potential as a therapeutic target in cancer where cell cycle dysregulation is prevalent. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562 cells treated with Insulin, using Phospho-p57 (Thr310) Rabbit Polyclonal Antibody (EAB10970) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
 Immunohistochemistry analysis of paraffin-embedded Human placenta tissue, using Phospho-p57 (Thr310) Rabbit Polyclonal Antibody (EAB10970) at 1:100 dilution.
 Immunofluorescence analysis of HuvEc cell, using Phospho-p57 (Thr310) Rabbit Polyclonal Antibody (EAB10970) at 1:100 dilution.
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