| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 63 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PDPK1 Rabbit Polyclonal Antibody detects endogenous levels of PDPK1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) also known as PDK1 is a 556 amino acid protein that is ubiquitously expressed and localizes to the cell membrane, cytoplasm, and nucleus, playing a crucial role in various cellular processes. As a master kinase, PDPK1 is essential for phosphorylating and activating a subgroup of the AGC family of protein kinases, which are pivotal in mediating signal transduction pathways that control cell proliferation, survival, and growth, particularly in developing pancreatic beta cells. Additionally, PDPK1 regulates calcium uptake and calcium-activated potassium channels in mast cells, influences chemotaxis and motility in vascular endothelial cells, and is vital for cardiac homeostasis and thymocyte development. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Mouse liver, CaSki cells, using PDPK1 Rabbit Polyclonal Antibody (EAB10939) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 63 kDa; Observed band size: 60-63 kDa  Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using PDPK1 Rabbit Polyclonal Antibody (EAB10939) at 1:100 dilution.
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