| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 15 | Human, Mouse, Rat, Monkey |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PEA-15 Rabbit Polyclonal Antibody detects endogenous levels of PEA-15 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PEA-15 is a death effector domain-containing protein that functions as a negative regulator of apoptosis. PEA-15 plays a crucial role in regulating apoptotic signaling pathways due to a unique structure, which includes a death effector domain (DED) within its first 80 amino acids. This DED is essential for PEA-15′s ability to inhibit Ras suppression, thereby influencing cell survival and apoptosis. PEA-15 exists in three forms: a non-phosphorylated form and two phosphorylated forms, which are regulated by protein kinase C (PKC). The phosphorylation status of PEA-15 changes in response to microtubule dynamics, indicating colocalization with these structures. While predominantly expressed in the central nervous system, PEA-15 is also found in lower levels in the liver and kidney, and at higher levels in muscle tissue. PEA-15 is also referred to as Phosphoprotein Enriched in Diabetes (PED) due to increased expression in individuals with type 2 diabetes, highlighting a potential role in metabolic regulation and disease. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells, using PEA-15 Rabbit Polyclonal Antibody (EAB10937) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using PEA-15 Rabbit Polyclonal Antibody (EAB10937) at 1:100 dilution.  Immunofluorescence blot analysis of extracts on Hela cell, using PEA-15 Rabbit Polyclonal Antibody (EAB10937) at 1:100 dilution.
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