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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 37 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-PP1α (Thr320) Rabbit Polyclonal Antibody detects endogenous levels of PP1α only when phosphorylated at Thr320. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PP1α also known as PP1A, PPP1CA is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1α is a critical serine/threonine-protein phosphatase localized predominantly in both the nucleus and cytoplasm of eukaryotic cells, where PP1α plays an essential role in regulating various cellular functions including cell division, homeostasis, and apoptosis through the dephosphorylation of target proteins. The precise localization of PP1α within the cell is vital for function, as PP1α ensures the correct modulation of phosphorylation states in specific cellular compartments, thereby maintaining cellular balance and responding to signaling cues. PP1α operates as part of the PP1 holoenzyme complex, interacting with regulatory and variable subunits to achieve substrate specificity and regulation. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells, using Phospho-PP1α (Thr320) Rabbit Polyclonal Antibody (EAB10931) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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