| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF, ELISA | 27 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-PRAS40 (Thr246) Rabbit Polyclonal Antibody detects endogenous levels of PRAS40 protein only when phosphorylated at Thr246. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PRAS40 also known as AKT1S1 is a proline-rich substrate of AKT that binds 14-3-3 protein when phosphorylated. PRAS40 plays a crucial role in cellular signaling pathways, particularly in the phosphatidylinositol 3-kinase pathway, which regulates various cellular processes including growth, survival, and metabolism. PRAS40 is primarily located in the cytoplasm, where PRAS40 interacts with other signaling molecules, including activated Akt. This interaction influences PRAS40′s phosphorylation state, which affects binding to 14-3-3 proteins. Such binding modulates PRAS40′s subcellular localization and regulates enzymatic activity, impacting cellular responses to growth factors and stress signals. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2 cells treated with PDGF, using Phospho-PRAS40 (Thr246) Rabbit Polyclonal Antibody (EAB10925) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 27 kDa; Observed band size: 40 kDa |
