| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 48 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Skp2 Rabbit Polyclonal Antibody detects endogenous levels of Skp2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Skp2 also known as Skp2 p45 is a member of the F-box protein family. Skp2 plays a pivotal role in regulating cell cycle progression, particularly in G1 to S phase transition, by targeting specific cyclins for degradation. Skp2 primarily resides in the nucleus, interacting with cyclin-dependent kinases and other regulatory proteins to facilitate timely cell cycle progression. Precise nuclear localization of Skp2 enables effective regulation of cyclin levels, maintaining cellular homeostasis and preventing uncontrolled cell proliferation. Dysregulation of Skp2 has been implicated in various cancers, highlighting Skp2′s importance as a potential therapeutic target. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat, NIH/3T3 cells, using Skp2 Rabbit Polyclonal Antibody (EAB10852) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human right kidney tissue, using Skp2 Rabbit Polyclonal Antibody (EAB10852) at 1:100 dilution.
 Immunofluorescence analysis of A549 cell, using Skp2 Rabbit Polyclonal Antibody (EAB10852) at 1:200 dilution.
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