| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IP, IHC-P, IF, ELISA | 91 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:20-200 IHC-P=1:50-300 IF=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Stat5a (Ser780) Rabbit Polyclonal Antibody detects endogenous levels of Stat5a only when phosphorylated at Ser780. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Stat5a also known as Signal Transducer and Activator of Transcription 5A is a critical transcription factor involved in the JAK-STAT signaling pathway, mediating cellular responses to a variety of cytokines and growth factors such as interleukins (e.g., IL-2, IL-3), erythropoietin, and growth hormone. Upon activation by phosphorylation through receptor-associated Janus kinases (JAKs), Stat5a dimerizes and translocates to the nucleus, where Stat5a binds specific DNA sequences to regulate gene expression involved in cell proliferation, differentiation, and apoptosis, especially in lymphohematopoietic cells. The proper functioning of Stat5a is essential for normal immune system development and function, and dysregulation has been implicated in various cancers and immune disorders. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells treated with PMA, using Phospho-Stat5a (Ser780) Rabbit Polyclonal Antibody (EAB10819) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
 Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma, using Phospho-Stat5a (Ser780) Rabbit Polyclonal Antibody (EAB10819) at 1:100 dilution. |
