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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P | 54 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-300 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-HS1 (Tyr397) Rabbit Polyclonal Antibody detects endogenous levels of HS1 protein when phosphorylated at Tyr397. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
HS1 also known as p75, LckBP1, HCLS1 is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin. HS1 plays a crucial role in T cell activation by associating directly with the Src homology 3 (SH3) domain of the Lck tyrosine kinase, which is essential for T cell receptor (TCR) signaling. This interaction is facilitated by two proline-rich regions within HS1, highlighting its importance in the modulation of T cell responses. Additionally, HS1 contains four tandem 37 amino acid repeats that form a putative helix-loop-helix DNA binding motif, suggesting a potential role in gene regulation following T cell activation. Notably, HS1 is tyrosine phosphorylated in T cells after TCR engagement, indicating its involvement in downstream signaling pathways that are critical for T cell proliferation and differentiation. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cell treated with TNF, using Phospho-HS1 (Tyr397) Rabbit Polyclonal Antibody (EAB10804) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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