| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IP, IHC-P, IF/ICC, ELISA | 15 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:100-500 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Histone H2A.X Rabbit Polyclonal Antibody detects endogenous levels of Histone H2A.X protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Histone H2A.X also known as X variant Histone H2A, H2AX is a member of the Histone H2A family involved in the nucleosomal organization of chromatin, differing from other H2A variants by a highly conserved C-terminal motif. Histone H2A.X plays a crucial role in the cellular response to DNA damage, particularly in the recognition and repair of double-stranded breaks. Upon exposure to ionizing radiation or other genotoxic stress, H2A.X is rapidly phosphorylated at Ser139, leading to the formation of distinct nuclear foci that serve as recruitment sites for DNA repair proteins, including Rad50, Rad51, and BRCA1. This phosphorylation event is vital for initiating the DNA damage response, as γ-H2A.X marks the sites of damage and facilitates the assembly of repair complexes, thereby maintaining genomic stability. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HepG2 cells, using Histone H2A.X Rabbit Polyclonal Antibody (EAB10788) at 1:2000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using Histone H2A.X Rabbit Polyclonal Antibody (EAB10788) at 1:100 dilution.
 Immunofluorescence analysis of Hela cell, using Histone H2A.X Rabbit Polyclonal Antibody (EAB10788)(red) at 1:200 dilution.
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