| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 147 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-HER4/ErbB4 (Tyr1284) Rabbit Polyclonal Antibody detects endogenous levels of HER4/ErbB4 protein only when phosphorylated at Tyr1284. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
ErbB4 also known as HER4 is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. ErbB4 plays a crucial role in cell signaling pathways that regulate cell proliferation, differentiation, and survival. ErbB4 is primarily located on the cell membrane, where ErbB4 can form homodimers or heterodimers upon ligand binding, a process essential for activation and subsequent signaling. ErbB4′s membrane localization enables interaction with various ligands, such as heregulin, which promotes proteolytic cleavage and ectodomain release. This ectodomain can then engage in paracrine signaling, influencing neighboring cells and contributing to tissue homeostasis and development. |
| Product Image Gallery | |
 Western blot analysis of extracts on HuvEc cells treated with EGF, using Phospho-HER4/ErbB4 (Tyr1284) Rabbit Polyclonal Antibody (EAB10787) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 147 kDa; Observed band size: 180 kDa |
