| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 67 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-GRB10 (Tyr67) Rabbit Polyclonal Antibody detects endogenous levels of GRB10 protein only when phosphorylated at Tyr67. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
GRB10 belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. GRB10 interacts with several receptor tyrosine kinases, which are essential for mediating cellular responses to growth factors. GRB10 regulates insulin signaling and has been implicated in metabolic processes, making GRB10 significant in studying conditions such as obesity and diabetes. GRB10 undergoes serine phosphorylation in response to epidermal growth factor treatment, while showing limited binding to the EGF receptor, highlighting complex regulatory mechanisms and potential as a therapeutic target. Mapping to mouse chromosome 11, GRB10 is located near the EGF receptor, suggesting a functional relationship that may influence receptor signaling dynamics. |
| Product Image Gallery | |
 Western blot analysis of extracts on NIH/3T3 cells treated with Insulin, using Phospho-GRB10 (Tyr67) Rabbit Polyclonal Antibody (EAB10781) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
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