| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IP, IHC-P, IF, ELISA | 45 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:20-200 IHC-P=1:50-300 IF=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Elk-1 (Ser383) Rabbit Polyclonal Antibody detects endogenous levels of Elk-1 protein only when phosphorylated at Ser383. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Elk-1 is a nuclear target for the ras-raf-MAPK signaling cascade. Elk-1 produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. Located predominantly in the nucleus, Elk-1′s nuclear localization is essential for binding to DNA and modulating the transcription of immediate early genes such as c-Fos, which are pivotal in cellular responses to growth signals and MAPK/ERK signaling pathways. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells treated with UV, using Phospho-Elk-1 (Ser383) Rabbit Polyclonal Antibody (EAB10733) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 45 kDa; Observed band size: 60 kDa  Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue, using Phospho-Elk-1 (Ser383) Rabbit Polyclonal Antibody (EAB10733) at 1:100 dilution.
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