| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 85 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-cPLA2 (Ser505) Rabbit Polyclonal Antibody detects endogenous levels of cPLA2 protein only when phosphorylated at Ser505. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
cPLA2 also knonwn PLA2G4A is a member of the cytosolic phospholipase A2 group IV family. cPLA2 plays a crucial role in the hydrolysis of membrane phospholipids, specifically targeting the sn-2-acyl ester bond. This enzymatic activity is vital for the release of arachidonic acid, a key precursor in the biosynthesis of eicosanoids, which include potent inflammatory mediators such as prostaglandins and leukotrienes. cPLA2 protein generates these bioactive lipids essential for various physiological processes, including inflammation, pain response, and immune regulation. cPLA2 protein is predominantly located in the cytosol of various cell types, including platelets, macrophages, and monoblasts, where activation occurs in response to increases in intracellular calcium levels. |
| Product Image Gallery | |
 Western blot analysis of lysates ok HuvEc cells treated with TNFalpha, using Phospho-cPLA2 (Ser505) Rabbit Polyclonal Antibody (EAB10700) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 85 kDa; Observed band size: 110 kDa  Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue, using Phospho-cPLA2 (Ser505) Rabbit Polyclonal Antibody (EAB10700) at 1:100 dilution.
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