| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 68 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-A-Raf (Tyr302) Rabbit Polyclonal Antibody detects endogenous levels of A-Raf protein only when phosphorylated at Tyr302. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
A-Raf also known as PKS2, ARAF1 is a member of the Raf gene family of serine/threonine protein kinases, plays a crucial role in cell signaling pathways that regulate cell growth and differentiation. A-Raf enables research into cancer biology, where aberrant activation of A-Raf can lead to uncontrolled cell proliferation and tumorigenesis. A-Raf is predominantly expressed in urogenital tissues and kidney, with lower expression levels in brain, highlighting potential roles in organ-specific signaling pathways. A-Raf kinase activity maintains tight regulation, and interaction with other signaling molecules, such as ERK/MAP kinases, underscores significance in mediating cellular responses to growth factors and stress signals. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela cells treated with PMA, using Phospho-A-Raf (Tyr302) Rabbit Polyclonal Antibody (EAB10625) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
 Immunohistochemistry of paraffin-embedded Human breast carcinoma tissue, using Phospho-A-Raf (Tyr302) Rabbit Polyclonal Antibody (EAB10625) at 1:100 dilution.
 Immunofluorescence analysis of HepG2 cell, using Phospho-A-Raf (Tyr302) Rabbit Polyclonal Antibody (EAB10625) at 1:100 dilution.
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