| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 49 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | RUNX1/AML1 Rabbit Polyclonal Antibody detects endogenous levels of RUNX1/AML1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
RUNX1 also known as AML1, CBFA2 is a crucial member of the Runt-related transcription factor family. RUNX1 plays a critical role in hematopoiesis, the process of blood cell formation, and is essential for proper development of various blood cell lineages. RUNX1 regulates the expression of genes involved in cellular differentiation and cell cycle progression, making RUNX1 a key player in maintaining normal hematopoietic function. RUNX1 is frequently implicated in human leukemia due to chromosomal translocations that disrupt DNA-binding domain, leading to aberrant gene expression and oncogenesis. Beyond hematopoiesis, RUNX1 is crucial for sensory neuron diversification, promoting axonal growth and mediating TrkA transactivation in neural crest-derived sensory neurons. Alternative splicing of RUNX1 results in several isoforms, further diversifying functional roles in cellular processes. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells, using RUNX1/AML1 Rabbit Polyclonal Antibody (EAB10616) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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